Proteonano™ and Evosep Integration for Automated High-Throughput Proteomics Workflow
Combining the Proteonano™ and Evosep platforms in an automated workflow delivers high throughput and stability, supporting large-scale proteomics for thousands of samples.
Using the Proteonano™ Ultraplex Proteomics Platform for automated enrichment of low-abundance proteins in plasma samples, specifically from healthy pool plasma.
Subsequently, the Evosep One system, coupled with the timsTOF Pro2, performs standard gradient mass spectrometry analysis. The overall process is outlined in the schematic diagram below:
Identification Number:
The results demonstrated the detection of 2,463 proteins with a 300 ng sample amount under a 30 SPD gradient condition. This is comparable to the identification of approximately 2,800 proteins using the same samples on the timsTOF Pro 2 with a 24 SPD (60 min) gradient.
Coverage of HPPP (Human Plasma Proteome Database) ratio:
The timsTOF Pro 2 detected proteins covering 1,733 proteins from the HPPP database, with 50% of these proteins having concentrations below 10 ng/mL. Additionally, 766 proteins
were identified beyond the HPPP database.
Dynamic range:
The proteome dynamic range detected in this experiment spanned over 9 orders of magnitude, representing nearly a 10,000-fold increase in identification depth compared to traditional methods. As shown in Figure 5, several proteins associated with aging and neurodegenerative diseases such as Alzheimer's disease were found to have concentrations below 10 ng/mL.
